Pharmacy Department
Aseptic Skill Validation Exercises
A pass in aseptic skill validation requires all 10 bags and all
16 syringes to show no growth at the end of incubation period.
Exercise 1
1. Using a 50ml syringe, withdraw and remove 100ml of
saline from soft bag provided.
2. Inject the following into the soft bag:-
| a. | Drug A (conc.10mg/ml), 800mg | = _____________ml |
| b. | Drug B (conc. 20mg/ml), 600mg | = _____________ml |
| c. | Drug C (conc. 25mg/ml), 60mg | = _____________ml |
| d. | Drug D (conc. 5mg/ml), 30mg | = _____________ml |
| e. | Drug E (conc. 1mg/ml), 50mg | = _____________ml |
3. Repeat step 1 and 2 for another 9 soft bags.
4. Label the bags according to institution standards.
Exercise 2
1. Using appropriate syringes, prepare the following
bolus injections from Drug X (conc. 50mg/ml)
:-
| a. | 350mg | = _____________ml |
| b. | 500mg | = _____________ml |
| c. | 600mg | = _____________ml |
| d. | 650mg | = _____________ml |
| e. | 700mg | = _____________ml |
| f. | 800mg | = _____________ml |
| g. | 1000mg | = _____________ml |
| h. | 1200mg | = _____________ml |
2. Label the syringes according to practice standards.
3. Repeat step 1 and 2 for 1 more time.

